Global Immobilized Trypsin Market
Drivers
Leading manufacturers are
involved in manufacture of immobilized trypsin enzyme with the reduced time of
incubation in the global Immobilized
Trypsin Market. For example, the immobilized trypsin manufactured by Geno
Technology Inc. and Promega Corporation requires a sample to be incubated in
the spin columns for 2-18 hrs. And for 30 mins at 37 degrees celsius,
respectively. In case of, Merck KGaA manufactured product, trypsin spin columns
with immobilized trypsin, the sample has to be incubated in the columns for 15
mins at room temperature. Therefore, the incubation time of the sample with the
immobilized trypsin has been decreased in case of Merck KGaA manufactured
product. This can benefit the researchers by rapid protein digestion for
protein analysis.
Immobilized trypsin finds many
applications in fundamental research. For instance, it is used in affinity
chromatography for the purification of trypsin inhibitors from the solution.
Immobilized trypsin finds many applications in research for studying protein –
protein interactions as well as protein identification in systems biology to
identify drug targets in the cells. Therefore, use of immobilized trypsin
offers an advantage in scientific research as the technique is cost-effective.
Apart from these applications, immobilized trypsin is used in other fields such
as to identify the post translational modifications. Other advantages of using
immobilized trypsin are, easy to use, wide spread availability, and high
specificity. The above factors are expected to boost the global immobilized
trypsin market growth over the forecast period.
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Trypsin is a serine protease
enzyme (EC number of 3.4.21.4), which hydrolyses esters of basic amino acids
such as lysine and arginine in the peptides. Optimal temperature for trypsin
activation is around 37 degrees Celsius and its optimal pH is around 7.5 to
9.0. Trypsin is available in the form of immobilized enzyme where it is
attached to an inert support such as dextran, sephadex, cellulose, starch etc.
Immobilized Trypsin is supplied in preservatives such as glycerol and sodium
azide as a slurry. It finds applications
in research for the purpose of protein identification, protein
characterization, study of post translational modifications, and protein-protein
interactions.
There are many manufacturers
producing immobilized trypsin like Thermo Fischer Scientific who manufactures
immobilized trypsin, treated with L-1-tosylamido-2-phenylethyl chloromethyl
ketone (TPCK) to inhibit chymotrypsin activity without altering the activity of
trypsin, in order to prevent contamination. Some of the other manufacturers are
Promega Corporation, Merck KGaA, etc.
Global Immobilized Trypsin Market
Restraints
According to the National Center
for Biotechnology Information (NCBI), 2017, disadvantages of using trypsin are
autolysis, intolerance to high temperatures or organic solvents and missing out
on particular sites of post-translational modifications, protein segments or
even subsets of proteins. To overcome this drawback, other enzymes are being
tested for the catalytic function, which would act as an alternatives for
immobilized trypsin. For instance, according to National Centre for
Biotechnology Information, (NCBI), 2016, the catalytic function of the six
proteases (Chemotrypsin, LysC, LysN, AspN, GluC and ArgC,) was researched and
the proteases have been shown to offer promising results. The above factors are
expected to restrain the global immobilized trypsin market growth over the
forecast period.
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Global Immobilized Trypsin Market
Regional Analysis
North America is expected to
witness significant growth in the global immobilized trypsin market over the
forecast period, owing to the manufacture of modified immobilized trypsin
enzyme with increased throughput and confidence in results. For instance, in
2015, Thermo Fisher Scientific launched, Thermo Scientific Digest Kit, a
protein analysis kit useful for applications related to biopharmaceuticals. The
kit includes a thermostable immobilized trypsin for reducing digestion time and
for reproducing high-quality data.
Moreover, headquarters of key
manufacturers have wide-spread presence in the North America region. For
instance, Promega Corporation, Merck KGaA, and Thermo Fisher Scientific are
located in the U.S. Therefore, the above factors are expected to boost the
global immobilized enzymes market growth over the forecast period.
Global Immobilized Trypsin Market
Key Players
Some of the key players operating
in the global immobilized trypsin market are Promega Corporation, Merck KGaA,
Geno Technology Inc., and Thermo Fisher Scientific
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